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动物组织DNA的提取及核酸浓度的测定

来源:学生作业帮 编辑:搜搜做题作业网作业帮 分类:英语作业 时间:2024/05/24 05:26:23
动物组织DNA的提取及核酸浓度的测定
为什么要测310nm处的吸光值?细胞裂解液和沉淀液中的EDTA 有什么作用?氯化钠和醋酸钠有什么作用?
动物组织DNA的提取及核酸浓度的测定
DNA concentration is measured by OD260,protein usually is OD280.Some proteins have peak absorbency around 310 nm.Therefore it can be used for judging protein contamination.
EDTA is able to chelate Mg2+ (Magnesium) which is required for most DNase.After Mg2+ is chelated,DNase will not work,this help to preserve DNA..
Sodium acetate and sodium chloride can help to precipitate DNA.